Fig. 3

Hyperspectral analysis of AgNPs within macrophages collected from BAL fluid healthy and MetS mouse models. A Representative enhanced darkfield images of macrophages at 3, 7, 14, and 21 days following AgNP exposure at 50 µg from healthy and MetS mice not receiving RvD1 treatment. B Representative enhanced darkfield images of macrophages at 3, 7, 14, and 21 days following AgNP exposure from healthy and MetS mice receiving 400 ng RvD1 treatment. At least 1000 pixels of AgNPs were collected from mean spectra and then all spectra were normalized based on intensity for comparisons. White bar identifies 10 µm scaling. Representative images of internalized cells and spectral profiles of internalized AgNPs over the study time course can be found in Supplemental Fig. 2–4