Fig. 5
DDAH1 overexpression alleviates long-term PM2.5 exposure-induced pulmonary alveoli injury, fibrosis and inflammatory cell infiltration. After exposure for 6 months, serum ADMA levels (A) and cell numbers in BALF B were measured. C Representative lung sections from FA- or PM2.5-exposed WT and DDAH1-TG mice were stained with H&E (Scale bar = 100 μm; the blue arrows point to enlarged alveolar, the red arrows point to collapsed alveolar structure), Masson trichrome (Scale bar = 100 μm), and TUNEL kits (white arrows point to TUNEL positive cells, Scale bar = 20 μm). The relative collagenous fiber area (D) and TUNEL positive cell number E were quantified. In the lungs of FA- and PM2.5-exposed mice, ADMA (F), 3′-NT G and 4-HNE H were measured. I Lysates of lung tissue were examined by western blotting analysis. In Figure A–H, N = 5; in Figure I, N = 3; data are presented as the mean ± SD; *indicates p < 0.05, **indicates p < 0.01