Fig. 7
ADMA treatment promotes lung inflammation and oxidative stress in PM2.5-exposed mice. A Representative lung sections were stained with antibodies specific for neutrophils, F4/80, ICAM-1 and VCAM-1, and DHE. Scale bar = 50 μm. B–F The relative neutrophil- B or F4/80-positive C cell numbers, the levels of ICAM-1 (D) and VCAM-1 (E) and the relative fluorescence intensity of DHE F were quantified. G–J The ADMA (G), NOx (H), 3′-NT (I) and 4-HNE J levels in the lung tissue were measured. K Lung lysates were examined by Western blotting. In Fig. B–J, N = 5; in Fig. K, N = 3; data are presented as the mean ± SD; *indicates p < 0.05, **indicates p < 0.01