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Fig. 7 | Particle and Fibre Toxicology

Fig. 7

From: Polypropylene nanoplastic exposure leads to lung inflammation through p38-mediated NF-κB pathway due to mitochondrial damage

Fig. 7

a Representative images of mitochondrial (green) and mitochondrial ROS (red) stained PP-exposed A549 cells that were treated with 200 nM for 30 min. b Quantified staining intensities for mitochondrial ROS. c Total ROS production in PP-exposed A549 cells. d Inflammatory cytokine levels of TNF-α, IL-1β, and IL-6 in cell lysate of PP-exposed A549 cells. e Relative density analysis of TNF-α, IL-1β, and IL-6. f Cell viability assessment by MTT assay. Data were normalized against β-actin. Data are means ± SD (n = 3 per group). #P ≤ 0.05; ##P ≤ 0.01; ###P ≤ 0.001 vs. VC. Scale bar 20 μm

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