Biokinetics of subacutely co-inhaled same size gold and silver nanoparticles

Table 2 The organ distribution for gold after inhaled of AuNP at exposure 1 day (E−1), post-exposure observation 1 day (PEO-1), PEO-7 days and PEO-28 days (mean ± S.E; 4 animals of exposure 1 day; 5 animals of PEO-1, PEO-7 and PEO-28)

AuNP	Liver			Kidney			Spleen			Testis			Epididymis
AuNP	ng/g^A	Organ wt (g)^B	Au/organ (ng)^C	ng/g^A	Organ wt (g)^B	Au/organ (ng)^C	ng/g^A	Organ wt (g)^B	Au/organ (ng)^C	ng/g^A	Organ wt (g)^B	Au/organ (ng)^C	ng/g^A	Organ wt (g)^B	Au/organ (ng)^C
E−1	0.002 ± -	8.99 ± 0.137	0.017 ± -	0.231 ± 0.028	1.238 ± 0.033	0.286 ± 0.035	N/D	0.641 ± 0.086	N/D	0.032 ± 0.004	1.463 ± 0.062	0.048 ± 0.007	0.028 ± 0.008	0.280 ± 0.032	0.008 ± 0.004
PEO1	1.913 ± 0.125	10.471 ± 0.368	19.956 ± 1.170	14.312 ± 0.978	1.337 ± 0.025	19.158 ± 1.444	1.061 ± 0.113	0.676 ± 0.040	0.712 ± 0.113	0.261 ± 0.018	1.614 ± 0.041	0.422 ± 0.033	0.200 ± 0.018	0.539 ± 0.061	0.108 ± 0.011^b
PEO7	1.840 ± 0.250	10.408 ± 0.962	19.007 ± 2.621	15.331 ± 1.302	1.475 ± 0.144	22.965 ± 3.432	1.416 ± 0.308	0.723 ± 0.040	1.042 ± 0.308	0.212 ± 0.012	1.528 ± 0.085	0.327 ± 0.032	0.246 ± 0.036	0.515 ± 0.054	0.129 ± 0.023
PEO 28	1.473 ± 0.113	12.332 ± 0.426	18.062 ± 1.182	18.193 ± 2.918	1.712 ± 0.096	30.461 ± 4.466	0.920 ± 0.133	0.972 ± 0.068	0.907 ± 0.133	0.248 ± 0.032	1.741 ± 0.080	0.423 ± 0.045	0.264 ± 0.020	0.658 ± 0.024	0.173 ± 0.012

Co-exposure	Liver			Kidney			Spleen			Testis			Epididymis
E−1	0.034 ± 0.007	9.362 ± 1.021	0.341 ± 0.097	0.144 ± 0.040	1.226 ± 0.110	0.166 ± 0.044	N/D	0.589 ± 0.074	0.166 ± 0.044	0.032 ± 0.004	1.528 ± 0.044	0.049 ± 0.006	0.048 ± 0.011	0.296 ± 0.012	0.014 ± 0.006
PEO1	0.787 ± 0.174	9.237 ± 0.529	7.103 ± 1.371	4.990 ± 0.154	1.257 ± 0.037	6.278 ± 0.297^bb	0.406 ± 0.065	0.571 ± 0.023	0.230 ± 0.065^b	0.086 ± 0.005	1.638 ± 0.089	0.140 ± 0.009^b	0.094 ± 0.008	0.482 ± 0.058	0.045 ± 0.005
PEO7	0.700 ± 0.157	9.987 ± 0.919	6.433 ± 0.843	5.731 ± 0.723	1.300 ± 0.074	7.298 ± 0.694^c	0.363 ± 0.029	0.656 ± 0.043	0.238 ± 0.029^c	0.092 ± 0.015	1.611 ± 0.087	0.145 ± 0.017	0.091 ± 0.023	0.550 ± 0.051	0.048 ± 0.011
PEO 28	0.471 ± 0.045	13.905 ± 0.894	6.490 ± 0.565	6.534 ± 0.847	1.609 ± 0.053	10.404 ± 1.186	0.359 ± 0.058	1.054 ± 0.142	0.355 ± 0.058	0.115 ± 0.011	1.677 ± 0.021	0.193 ± 0.016	0.101 ± 0.010	0.675 ± 0.080	0.069 ± 0.008

AuNP	Thymus			Olfactory bulb			Hilar lymph node			Brachial lymph node			Brain
E−1	N/D	0.728 ± 0.060	N/D	0.314 ± 0.091	0.082 ± 0.004	0.026 ± 0.008	5.018 ± 1.434	0.019 ± 0.002	0.071 ± 0.021	0.416 ± 0.218	0.082 ± 0.004	0.032 ± 0.016	0.042 ± 0.019	2.053 ± 0.039	0.083 ± 0.038
PEO1	0.236 ± 0.053	0.418 ± 0.075	0.091 ± 0.015^b	5.899 ± 2.572	0.097 ± 0.013	0.512 ± 0.176	578.442 ± 153.265	0.013 ± 0.002	6.633 ± 1.147	6.967 ± 1.480	0.097 ± 0.013	0.666 ± 0.179	0.225 ± 0.085	2.113 ± 0.047	0.472 ± 0.181
PEO7	0.186 ± 0.067	0.408 ± 0.084	0.074 ± 0.025	3.999 ± 0.900	0.059 ± 0.013	0.219 ± 0.044	705.582 ± 375.512	0.029 ± 0.009	10.761 ± 1.180^c	5.637 ± 1.464	0.059 ± 0.013	0.372 ± 0.147	0.055 ± 0.026	2.197 ± 0.037	0.118 ± 0.055
PEO 28	0.052 ± 0.004	0.397 ± 0.022	0.023 ± 0.003	3.500 ± 0.480	0.059 ± 0.014	0.227 ± 0.075	156.841 ± 30.359	0.035 ± 0.004	5.724 ± 1.518	2.908 ± 0.754	0.059 ± 0.014	0.174 ± 0.060	0.198 ± 0.105	2.312 ± 0.041	0.465 ± 0.249

Co-exposure	Thymus			Olfactory bulb			Hilar lymph node			Brachial lymph node			Brain
E−1	N/D	0.573 ± 0.043	N/D	0.174 ± 0.135	0.061 ± 0.008	0.008 ± 0.006	2.042 ± 0.342	0.019 ± 0.005	0.018 ± 0.005	N/D	0.061 ± 0.008	N/D	N/D	2.093 ± 0.015	N/D
PEO1	0.142 ± 0.074	0.430 ± 0.055	0.043 ± 0.016	0.812 ± 0.135	0.103 ± 0.008	0.081 ± 0.011	211.322 ± 128.818	0.022 ± 0.005	3.216 ± 1.390	0.932 ± 0.448	0.103 ± 0.008	0.101 ± 0.051	0.086 ± -	2.079 ± 0.023	0.180 ± -
PEO7	0.013 ± -	0.498 ± 0.044	0.005 ± -	0.490 ± 0.179	0.108 ± 0.008	0.056 ± 0.023	57.529 ± 11.634	0.034 ± 0.004	1.840 ± 0.270	1.998 ± 0.686	0.108 ± 0.008	0.206 ± 0.077	0.029 ± -	2.118 ± 0.062	0.060 ± -
PEO 28	N/D	0.466 ± 0.044	N/D	0.511 ± 0.089	0.111 ± 0.008	0.058 ± 0.012	31.982 ± 6.204	0.050 ± 0.005	1.585 ± 0.362	N/D	0.111 ± 0.008	N/D	0.136 ± -	2.277 ± 0.032	0.309 ± -

A statistics analysis was used Anova and test.
A, samples were analyzed using by ICP-MS and calculated following ppb (ug/L) × final volume (5 mL) × dilution factor (10 times) / organ weight.
B, Organ weights were measured using by micro balance. Before measure weight, washed blood to di-water and wiped up to paper.
C, The gold concentration (ng/g) × organ weight (g); N/D was analyzed sample silver concentration lower than compare with control.
Statistics were analyzed Anova (multiple comparison analysis) and t-test. This result was described in Table that Anova was from a to cc and t-test was * and **.
^aP < 0.05, compared with PEO-1 and PEO-7
^bP < 0.05, compared with PEO-1 and PEO-28
^aaP < 0.01, compared with PEO-1 and PEO-7’
^bbP < 0.01, compared with PEO-1 and PEO-28
*P < 0.05, compared with PEO-1 and PEO-7
**P < 0.01, compared with PEO-1 and PEO-28

ISSN: 1743-8977