Establishing relationships between particle-induced in vitro and in vivo inflammation endpoints to better extrapolate between in vitro markers and in vivo fibrosis

Table 3 EC50 results for α-quartz in vivo PMN influx and in vitro inflammatory endpoints

Dose —cm²/cm²	IL-6 in vitro	TNF-α in vitro		in vivo
log(agonist) vs. normalized response—Variable slope	Damby et al. [47], A549, 24 h	Balduzzi et al. [52], RAW 246.7, 24 h	Nattrass et al. [51], J774, 24 h	PAR = 300cm²
Best-fit value, EC50	1.695	1.095	1.988	0.9603
95% CI, EC50	0.3925 to 6.761	1.708 to ???	0.8200 to 1.452	0.9513 to 0.9683
Best-fit value, HillSlope	3.433	2.471	Unstable	40.85

The in vitro inflammatory endpoints included in the table all showed overlap with in vivo PMN influx

ISSN: 1743-8977