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Fig. 3 | Particle and Fibre Toxicology

Fig. 3

From: Lung versus gut exposure to air pollution particles differentially affect metabolic health in mice

Fig. 3

Separate lung exposure to air pollution particles leads to lung inflammation, hypercholesterinemia and increased liver lipids in mice fed a standard diet. A Representative picture of lungs from mice intratracheally instilled with diesel exhaust particles (DEP), particulate matter (PM) or PBS. B Frequencies of lung monocytes and macrophages among CD11b+ cells (MHC II cells correspond to monocytes and macrophages; MHC II+ cells to CD11b+ DCs and resident interstitial macrophages). Eosinophils (SiglecF+CD11c+), alveolar macrophages (SiglecF+CD11b) and lung neutrophils (Ly6G+CD11b+) were gated on CD45+ cells (gating strategy Additional file 1: Fig. S2A). C Plasma TNF and IL-6. D Cholesterol, high-density lipoproteins (HDL), and triglycerides (TG). E Liver lipids. F Liver enzymes (alkaline phosphatase (AP), alanine transaminase (ALAT)). G Inflammatory gene expression of liver normalized to PBS. H Frequencies of adipose tissue macrophages (ATM), and their subpopulations defined by CD11c and CD206 and gene expression (gating Additional file 1: Fig. S2B). I Inflammatory gene expression in adipose tissue, normalized to PBS. Data are presented as mean ± SEM of 5 mice per group from one experiment compared by a two-tailed, unpaired Mann–Whitney U test (*p < 0.05)

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