Fig. 3From: Lung versus gut exposure to air pollution particles differentially affect metabolic health in miceSeparate lung exposure to air pollution particles leads to lung inflammation, hypercholesterinemia and increased liver lipids in mice fed a standard diet. A Representative picture of lungs from mice intratracheally instilled with diesel exhaust particles (DEP), particulate matter (PM) or PBS. B Frequencies of lung monocytes and macrophages among CD11b+ cells (MHC II− cells correspond to monocytes and macrophages; MHC II+ cells to CD11b+ DCs and resident interstitial macrophages). Eosinophils (SiglecF+CD11c+), alveolar macrophages (SiglecF+CD11b−) and lung neutrophils (Ly6G+CD11b+) were gated on CD45+ cells (gating strategy Additional file 1: Fig. S2A). C Plasma TNF and IL-6. D Cholesterol, high-density lipoproteins (HDL), and triglycerides (TG). E Liver lipids. F Liver enzymes (alkaline phosphatase (AP), alanine transaminase (ALAT)). G Inflammatory gene expression of liver normalized to PBS. H Frequencies of adipose tissue macrophages (ATM), and their subpopulations defined by CD11c and CD206 and gene expression (gating Additional file 1: Fig. S2B). I Inflammatory gene expression in adipose tissue, normalized to PBS. Data are presented as mean ± SEM of 5 mice per group from one experiment compared by a two-tailed, unpaired Mann–Whitney U test (*p < 0.05)Back to article page