Skip to main content
Fig. 7 | Particle and Fibre Toxicology

Fig. 7

From: ROS generation and p-38 activation contribute to montmorillonite-induced corneal toxicity in vitro and in vivo

Fig. 7Fig. 7

Effects of montmorillonite (Mt) on the MAPK signaling pathway in vitro and in vivo. A, B Representative bands of the western blot of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in the total proteins. Total proteins were extracted from HCEC-B4G12 cells exposed to various concentrations (1.56–100 μg/mL) of Na-Mt for 12 h and 24 h. C, D Quantification of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in A, B according to the densitometric analysis. Intensities of bands were normalized to the amount of GAPDH. *p < 0.05 between the Na-Mt treatment and the control. E–J Representative images indicating the expression of p-JNK (E, red), JNK (F, green), p-ERK1/2 (G, red), ERK1/2 (H, green), p-p38 (I, red), and p38 (J, green), in the cornea. Corneal sections were prepared from rats after exposing them to different concentrations (2 and 10 mg/mL) of Na-Mt or C-H-Na-Mt for 7 days. Nuclei were stained by DAPI (blue). (K, L) Quantified fluorescence intensity of the proteins in (E–J). *p < 0.05 compared with the control. Scale bars: 50 μm

Back to article page