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Fig. 8 | Particle and Fibre Toxicology

Fig. 8

From: ROS generation and p-38 activation contribute to montmorillonite-induced corneal toxicity in vitro and in vivo

Fig. 8

Effects of NAC pretreatment on the MAPK signaling pathway. A, B HCEC-B4G12 cells were pretreated with 10 μM SB203580 (p38 inhibitor) for 2 h prior to a treatment with Na-Mt for additional 24 h. The expression levels of p38 and p-p38 were investigated by western blot analysis (A). ATP content was measured by the CellTiter-Lum Plus Luminescent Cell Viability Assay (B). C Representative western bands of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in HCEC-B4G12. HCEC-B4G12 cells were pretreated with 10 mM NAC for 1 h before exposing them to 25 μg/mL Na-Mt for an additional 24 h. GAPDH is the loading control (A, C). Data points are the mean ± SD from three independent experiments, with three parallel samples per concentration in each experiment. *p < 0.05 compared with the control lacking the SB203580 pretreatment. #p < 0.05 compared with the control receiving the SB203580 pretreatment. &p < 0.05 between treatments with and without SB203580 pretreatment at the same concentration of Na-Mt

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