Fig. 2

T1D and DIO led to an enhanced PM-induced lung injury and systemic toxicity. (A) Representative H&E-stained images (scale bar, 100 μm) in different mouse models with or without PM exposure, with typical pathological changes marked, including neutrophil infiltration in the interstitial space (✦) or in the alveolar space (▲), and proteinaceous debris in the airspace (↖). (B) Representative images of TUNEL staining (scale bar, 50 μm), positive cells were marked as red fluorescent. (C) Acute lung injury (ALI) scores derived from Fig. 2A. (D) Quantitative analysis of cell apoptosis based on Fig. 2B. (E) The number of neutrophils in the peripheral blood (n = 10). Systemic oxidative stress was indicated by the content of plasma MDA (F) and plasma GSH (G). H. DNA damage indicated by quantitative analysis of olive tail moment from the Comet Assay. I. The heatmap based on plasma cytokines levels (IFN-γ, IL-1β, TNF-α, IL-17, IL-4, and IL-10) (All n = 5). The data are presented as mean ± SEM. ^*P < 0.05 (PM vs. AF); ^#P < 0.05 (DIO vs. ND); ^&P < 0.05 (T1D vs. ND); ^$P < 0.05 (T1D vs. DIO).