Fig. 5

Differential xenobiotic metabolism patterns in the lungs and livers of DIO and T1D mice. (A) Perturbations of key xenobiotic metabolism pathways before and after PM exposure in the livers of DIO or T1D mice compared with ND-fed mice. (B) The diagram presented dysregulated xenobiotic metabolism in lung tissues, exemplified by PM-induced changes in T1D mice compared to ND-fed mice. Pink elements and yellow arrows indicate the upregulation in T1D mice compared with ND-fed mice, while blue elements and arrows indicate the downregulation changes. Immunoblotting of AHR, CAR, and PXR in the lung (C) and liver (D). The data are presented as mean ± SEM. The heatmap showed the mRNA levels of phase I/II metabolizing enzymes and transporters in the lung (E) and liver (F). The z-score transformation was utilized to calculate the relative level of gene expression. (All n = 3)