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Fig. 2 | Particle and Fibre Toxicology

Fig. 2

From: Human dendritic cell maturation induced by amorphous silica nanoparticles is Syk-dependent and triggered by lipid raft aggregation

Fig. 2

Syk inhibition alleviates allogeneic T-cell response to SAS-NP-treated moDCs. Immature moDCs were pre-treated for one hour with Syk inhibitor IV (1 μM) and then treated for 16 h with 25 µg.mL−1 of SAS-NPs. Treated moDCs were co-cultured with allogeneic CD4+ T-cells loaded with CFSE at a ratio of 1 moDC for 20 CD4+ T-cells. A Proliferation was measured after 5 days of co-culture as the percentage of CFSElow CD4+ T-cells. Untreated moDCs were used as control. B On day 5, cytokine levels were quantified in co-culture supernatants in duplicate, using an electroluminescent multiplex assay. Detection limits are indicated in the Methods section. Results for lymphocyte proliferation are expressed as percentage of CFSE low cells. Cytokine production is expressed in pg.mL−1. Both are represented as the mean ± SEM of 6 independent experiments. *p < 0.05 One-way Anova and Kruskal–Wallis test

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