Fig. 4

JNK and p38 MAPK are phosphorylated in response to SAS-NPs. A and B. Immature moDCs were treated for 30, 60, 90 and 120 min with SAS-NPs (12.5 or 25 μg.mL⁻¹). A Representative experiment. B Immunoblotting of whole-cell extracts was used to quantify the phosphorylated forms of JNK and p38 kinases. C and D. Immature moDCs were pre-treated for 1 h with Syk inhibitor IV (1 μM) and then treated for 90 or 120 min with 25 µg.mL⁻¹ of SAS-NPs. C Representative experiment. D Immunoblotting of whole-cell extracts was used to quantify the phosphorylated forms of JNK and p38. The results of 3 independent experiments are represented. Bands were quantified using the Image Lab software. Results are expressed as the fold induction, representing the ratio of the normalised intensity (pJNK/actin or pP38/actin) of specific bands of treated cells divided by the normalised intensity of bands of untreated cells, *p < 0.05 One-way Anova, Kruskal-Wallis test.