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Fig. 6 | Particle and Fibre Toxicology

Fig. 6

From: MMP-3-mediated cleavage of OPN is involved in copper oxide nanoparticle-induced activation of fibroblasts

Fig. 6

Activation of MRC-5 fibroblasts cultured in the conditioned media from Nano-CuO-exposed BEAS-2B or U937* cells. a is the experimental protocol. BEAS-2B or U937* cells were treated with 1 µg/mL of Nano-CuO for 12 h and the conditioned media (CM) were collected to culture MRC-5 fibroblasts. After 0, 12, 24, 48, and 72 h culture, the MRC-5 fibroblasts were collected for protein isolation and Western blot. b and d were the results of a single Western blot experiment. c and e were the average expression levels of α-SMA, Col1A1, and fibronectin normalized to β-actin from three independent experiments. Data represent mean ± SE (n = 3). * Significant difference as compared to the control group, p < 0.05. Graphics in a were created with BioRender (https://BioRender.com)

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