Figure 3From: Silica induces NLRP3 inflammasome activation in human lung epithelial cells Silica augments processing and secretion of inflammatory mediators and DAMPs in bronchial epithelial cells. A. Western blot analysis was conducted on whole cell lysates of BEAS-2B cells to detect cleaved IL-1β products, after 24 h of silica exposure with or without priming with 5μg/mL LPS for 4h. Histogram represents results of densitometric analysis of cleaved IL-1β normalized to β-actin. B. Western blot analysis of HMGB1 release in the concentrated SN from silica exposed BEAS-2B cells. Histogram represents results of semi-quantitative densitometric analysis of the HMGB1 signal expressed as arbitrary units. Data are presented as mean fold change ± SEM with * p-value <0.05 compared to UC and # p-value <0.05 compared to SIL150 or LPS+SIL150.Back to article page