Figure 3From: Titanium dioxide nanoparticle impact and translocation through ex vivo, in vivo and in vitro gut epitheliaNP impact on cell viability and epithelial integrity. Cell viability probed with the MTT assay (A) or trypan blue exclusion assay (B) after 48 h of exposure to TiO2-NP concentrations ranging from 0 to 200 μg/mL. Results are expressed as average of eight replicates (MTT) or three replicates (trypan blue) ± standard deviation; they were considered statistically significant (*) when p < 0.05. Caco: Caco-2, HT29: HT29-MTX. Assessment of apoptosis and necrosis in control epithelia or epithelia exposed for 48 h to 50 μg/mL of TiO2-NPs (C): cells were stained with acridin orange/ethidium bromide; viable cells stain green, apoptotic cells stain orange and necrotic cells stain red.Back to article page