Figure 6From: Cytokine responses of human lung cells (BEAS-2B) treated with micron-sized and nanoparticles of metal oxides compared to soil dustsHigh surface area particles can interfere with measurement of cytokines in cell-free media containing a known aliquot of recombinant IL-6. A. Addition of 200 μg/mL of nano-SiO2, nano-TiO2, or kaolin to KGM media with the indicated concentration of BSA added. B. Addition of 200 μg/mL of nano-SiO2, nano-TiO2, or kaolin to KGM media with the indicated concentration of bovine serum added. C. The effect of adding increasing amounts of the indicated particles to KGM media without supplemental protein. Measured IL-6 divided by standard, * indicates significantly less than standard prepared in assay diluent, # indicates significantly greater than the IL-6 in the same particle suspension in media without exogenous protein addition, (two-tailed, p < 0.05). N = 3.Back to article page