Figure 3
Immunofluorescence labeling of F-actin after NPs exposure. Representative micrographs using F-actin phalloidin FITC antibodies under epifluorescence microscope (Olympus BH2, magnification ×400) after 24 h exposure of NPs: control (A: IP15 cells, B: LLC-PK1 cells) – FW2 (C and D:1 μg/cm2, F and G:10 μg/cm2 and I and J: 40 μg/cm2 on IP15 and LLC-PK1 cells, respectively) – TiO2-15 (E: 1 μg/cm2, H: 10 μg/cm2 and K: 40 μg/cm2 on LLC-PK1 cells).