Figure 4

Exposure to DEP prior to infection with influenza increases tissue eosinophils levels in OVA-sensitized mice. Lung sections stained for eosinophils using Carbol Chromotrope were analyzed for the presence of perivascular and peribronchial eosinophils. A.) Average numbers of tissue eosinophils located around small airways (150–200 μm) and blood vessels (10–50 μm). B-E.) Representative images from an animal exposed to DEP prior to infection with influenza illustrating the number of infiltrated eosinophils around the airways and blood vessel. Images illustrating peribronchial accumulation of eosinophils (B + C) at 40× (B) and 100× (C) magnification and perivascular accumulation of eosinophils (D + E) at 40× (D) and 100× (E) magnification. Br = bronchi; Ep = epithelium; BV = blood vessel, arrows point to individual eosinophils. *significantly different from Sal/Sal control; p < 0.05.