Figure 2

TLR2 activation modulates protein expression of SR-A1 in MDDC. MDDC were cultivated with TLR2, -3 and -4 ligands respectively Pam₃CSK₄ (10 μg/ml), poly(I:C) (10 μg/ml) and LPS (1 μg/ml) during 8 h for the mRNA expression (part A) and 24 h for the protein membrane expression (part B). A- MDDC were harvested for mRNA isolation followed by measurement of SR-A1 mRNA levels by quantitative RT-PCR. Results were expressed as the mean ± SEM of the relative gene expression calculated for each experiment in folds (2^-(ΔΔCt)) compared to unstimulated cells used as calibrator (n = 5). B- Dendritic cells were labeled for SR-A1, and analyzed by flow cytometry. Data are expressed as the mean ± SEM from 5 independent experiments. *: p < 0.05; **: p < 0.01 compared with cells in medium alone.