Table 8 Histopathological findings for male rats after 90-day oral administration of silver nanoparticles
GROUP | Control | Low | Middle | High | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Number of animals | 10 | 10 | 10 | 10 | |||||||
| Â | N | % | N | % | N | % | N | % | |||
Liver | No microscopic findings | 5/10 | 50 | 3/10 | 30 | 0/10 | 0 | 3/10 | 30 | ||
| Â | Abnormality | 5/10 | 50 | 7/10 | 70 | 10/10 | 100 | 7/10 | 70 | ||
|  |    Hyperplasia | bile duct | minimum | 4/10 | 40 | 7/10 | 70 | 8/10 | 80 | 6/10 | 60 |
| Â | Â | Â | mild | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 | 0/10 | 0 |
|  |    Vacuolation | hepatocellular | minimum | 1/10 | 10 | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 |
|  |    Necrosis | focal | minimum | 0/10 | 0 | 4/10 | 40 | 3/10 | 30 | 4/10 | 40 |
| Â | Â | lobular | moderate | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 | 0/10 | 0 |
| Â | Â | multifocal | moderate | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 | 0/10 | 0 |
|  |    Hemorrhage |  |  | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 | 0/10 | 0 |
|  |    Pigmentation |  |  | 0/10 | 0 | 1/10 | 10 | 0/10 | 0 | 2/10 | 20 |
Kidneys | No microscopic findings | 10/10 | 100 | 10/10 | 100 | 9/10 | 90 | 6/10 | 60 | ||
| Â | Abnormality | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 | 4/10 | 40 | ||
|  |    Basophilia | tubular | minimum | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 | 2/10 | 20 |
|  |    Inflammation | focal | minimum | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 |
| Â | Â | tubular | minimum | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 |
Lungs | No microscopic findings | 9/10 | 90 | 10/10 | 100 | 9/10 | 90 | 7/10 | 70 | ||
| Â | Abnormality | 1/10 | 10 | 0/10 | 0 | 1/10 | 10 | 3/10 | 30 | ||
|  |    Inflammation | focal | minimum | 1/10 | 10 | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 |
|  |    Histocytosis |  |  | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 | 0/10 | 0 |
|  |    Mineralization |  | minimum | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 2/10 | 20 |
|  |    Anthracosis |  |  | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 |
Intestines | No microscopic findings | 10/10 | 100 | 10/10 | 100 | 2/10 | 20 | 2/10 | 20 | ||
| Â | Abnormality | 0/10 | 0 | 0/10 | 0 | 8/10 | 80 | 8/10 | 80 | ||
|  |    Pigment | villi | yellow | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 | 8/10 | 80 |
| Â | Â | Â | faint yellow | 0/10 | 0 | 0/10 | 0 | 7/10 | 70 | 0/10 | 0 |
Heart | No microscopic findings | 10/10 | 100 | 10/10 | 100 | 8/10 | 80 | 8/10 | 80 | ||
| Â | Abnormality | 0/10 | 0 | 0/10 | 0 | 2/10 | 20 | 2/10 | 20 | ||
|  |    Inflammation | left ventricle | minimum | 0/10 | 0 | 0/10 | 0 | 2/10 | 20 | 1/10 | 10 |
| Â | Â | right ventricle | minimum | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 |
Eyes | No microscopic findings | 10/10 | 100 | 10/10 | 100 | 10/10 | 100 | 9/10 | 90 | ||
| Â | Abnormality | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 | ||
|  |    Inflammation | Harderian gland | minimum | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 1/10 | 10 |
Pancreas | No microscopic findings | 10/10 | 100 | 10/10 | 100 | 10/10 | 100 | 8/10 | 80 | ||
| Â | Abnormality | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 2/10 | 20 | ||
|  |    Inflammation | pancreas | minimum | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 2/10 | 20 |