Problems and challenges in the development and validation of human cell-based assays to determine nanoparticle-induced immunomodulatory effects

Table 3 RNA recovery and integrity from human monocytes exposed to NPs

^a Human monocytes (3-5 × 10⁶ cells/well) were exposed for 24 h to culture medium alone or to 0.6-4.9 μg/ml (corresponding to 4.55% v/v) of different NPs or their solvents.
^b RNA recovery in control cells varied between 700 and 1400 ng/10⁶ cells in seven separate experiments. Recovery in NP-treated cells is calculated as percent of the recovery in control cells (exposed to culture medium alone) within each single experiment, and expressed as mean ± SD. Recovery in cells treated with AgO NPs, tested in four experiments, was highly variable.
^c A value of RIN (RNA Integrity Number) between 7 and 10 indicates high quality intact RNA.
* p < 0.05 vs. medium control

ISSN: 1743-8977