Cytotoxicity screening of 23 engineered nanomaterials using a test matrix of ten cell lines and three different assays

Table 2 Interference of nanomaterials with in vitro toxicity test systems

	Parameters and particle condition
	Oxidative stress				Metabolic activity				Cell death
	DCF fluorescence				MTT light absorption				INT light absorption
	Particle dispersion on cells [μg cm ^-2 ]		Particles on cells after washing [μg cm ^-2 ]		Particle dispersion on cells [μg cm ^-2 ]		Particles on cells after washing [μg cm ^-2 ]		Particle dispersions cell free [μg cm ^-2 ]
Particles	10	50	10	50	10	50	10	50	10	50
AlOOH I	+	+	-	+	+	+	-	+	-	+
AlOOH II	+	+	-	+	+	+	-	+	-	+
Al-Ti-Zr 1	+	+	-	+	+	+	-	+	-	+
Al-Ti-Zr 2	+	+	-	+	+	+	-	+	-	+
Al-Ti-Zr 3	+	+	-	+	+	+	-	+	-	+
BaSO ₄	+	+	-	+	+	+	-	+	-	+
Carbon Black	+	+	(+)	+	+	+	+	+	-	+
CeO ₂	+	+	-	+	+	+	-	+	-	+
CeO ₂ A	+	+	-	+	+	+	-	+	-	+
CeO ₂ B	+	+	-	+	+	+	-	+	-	+
CeO ₂ C	+	+	-	+	+	+	-	+	-	+
CeO ₂ D	+	+	-	+	+	+	-	+	-	+
SrCO ₃ 1	+	+	-	+	+	+	-	+	-	+
SrCO ₃ 2	+	+	-	+	+	+	-	+	-	+
TiO ₂ 2	+	+	-	+	+	+	-	+	-	+
TiO ₂ 1	+	+	-	+	+	+	-	+	-	+
TiO ₂ 3	+	+	-	+	+	+	-	+	-	+
Ti-Zr 1	+	+	-	+	+	+	-	+	-	+
Ti-Zr 2	+	+	-	+	+	+	-	+	-	+
Ti-Zr 3	+	+	-	+	+	+	-	+	-	+
ZrO ₂ 1	+	+	-	+	+	+	-	+	-	+
ZrO ₂ 2	+	+	-	+	+	+	-	+	-	+
ZrO ₂ 3	+	+	-	+	+	+	-	+	-	+

optical interference. - no interference detected; (+) slight interference detected; + interference detected

ISSN: 1743-8977