Figure 2
In-vivo study: Effect of UfCP inhalation on pro-inflammatory (left) and anti-inflammatory (right) endogenous lipid mediator levels in lung tissue of non sensitized and sensitized mice. Non sensitized mice were exposed to UfCP for 24 h (UfCP group) or kept in filtered air (untreated group). OVA sensitized mice were exposed either to filtered air for 24 h and subsequently challenged with OVA (S/OVA group), or to UfCP for 24 h and then challenged with OVA (S/UfCP/OVA group). At day 0, 1 and 7 after particle exposure/OVA challenge the lungs were excised after BAL and used for analysis. The results are expressed in pg lipid mediator/mg protein analysed by specific enzyme immunoassays: Left panel: LTB4 and 8-isoprostane (8-isoprostane reprinted with permission from 22); right panel: PGE2, 15(S)-HETE and LXA4. Baseline values of untreated mice (dotted lines) are for LTB4: 264 ± 34 pg/mg; 8-isoprostane: 845 ± 231 pg/mg; PGE2: 34284 ± 4628 pg/mg; 15(S)-HETE: 1699 ± 189 pg/mg; LXA4: 125 ± 54 pg/mg. The data are given as absolute values (mean ± SD), n = 4-9/time point; *p <0.05 and **p <0.01 vs baseline; +p <0.05 and ++p <0.01 vs S/UfCP/OVA.