Silica-induced NLRP3 inflammasome activation in vitro and in rat lungs

Peeters, Paul M; Eurlings, Irene M J; Perkins, Timothy N; Wouters, Emiel F; Schins, Roel P F; Borm, Paul J A; Drommer, Wolfgang; Reynaert, Niki L; Albrecht, Catrin

doi:10.1186/s12989-014-0058-0

Particle and Fibre Toxicology

Table 1 Summary of histopathological evaluation of lung tissue of rats exposed to DQ12, DQ-PVNO or PBS

From: Silica-induced NLRP3 inflammasome activation in vitro and in rat lungs

Alveolar lipoproteinosis				Mixed cell alveolar inflammation
	PBS	DQ12	DQ12-PVNO		PBS	DQ12	DQ12-PVNO
3d	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	3d	0.0 ± 0.0	1.2 ± 0.7*	0.0 ± 0.0^#
7d	0.0 ± 0.0	0.2 ± 0.4	0.0 ± 0.0	7d	0.0 ± 0.0	1.1 ± 0.7*	0.5 ± 0.6
28d	0.0 ± 0.0	1.0 ± 0.3**	0.3 ± 0.4^##	28d	0.0 ± 0.0	0.8 ± 1.1	0.0 ± 0.0
90d	0.0 ± 0.0	1.3 ± 0.8*	0.0 ± 0.0^#	90d	0.0 ± 0.0	0.5 ± 0.4	0.1 ± 0.2
180d	0.0 ± 0.0	2.0 ± 0.4**	0.3 ± 0.4^##	180d	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0
360d	0.0 ± 0.0	2.6 ± 1.5**	2.9 ± 1.5^$$	360d	0.0 ± 0.0	0.6 ± 1.0	0.0 ± 0.0
Alveolar histocytosis				Brochoalveolar hyperplasia
	PBS	DQ12	DQ12-PVNO		PBS	DQ12	DQ12-PVNO
3d	0.0 ± 0.0	0.8 ± 0.7	0.1 ± 0.3	3d	0.0 ± 0.0	0.7 ± 0.9	0.3 ± 0.7
7d	0.3 ± 0.7	1.5 ± 0.7	0.5 ± 0.4^#	7d	0.9 ± 1.8	2.2 ± 1.4	0.6 ± 0.8
28d	1.0 ± 0.4	3.4 ± 1.4*	1.4 ± 0.8^#	28d	1.0 ± 0.4	3.1 ± 1.8	1.4 ± 1.2
90d	0.4 ± 0.7	3.8 ± 0.6**	1.0 ± 0.7^##	90d	0.8 ± 1.0	2.6 ± 1.4	0.6 ± 0.3
180d	0.8 ± 0.4	2.4 ± 0.6*	2.5 ± 1.0 ^$	180d	0.1 ± 0.3	2.3 ± 1.0**	2.3 ± 0.7 ^$$
360d	0.3 ± 0.3	3.0 ± 0.6**	2.0 ± 0.4 ^$$,#	360d	0.0 ± 0.0	5.5 ± 1.1**	3.9 ± 1.0 ^$$,#
Fibrosis alveolar septa				Perivascular fibrosis
	PBS	DQ12	DQ12-PVNO		PBS	DQ12	DQ12-PVNO
3d	0.0 ± 0.0	0.1 ± 0.3	0.0 ± 0.0	3d	1.0 ± 0.2	1.0 ± 0.0	0.9 ± 0.2
7d	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	7d	0.6 ± 0.2	1.0 ± 0.0*	1.0 ± 0.0
28d	0.0 ± 0.0	0.4 ± 0.6	0.0 ± 0.0	28d	1.1 ± 0.2	1.3 ± 0.4	1.1 ± 0.2
90d	0.0 ± 0.0	1.0 ± 0.8*	0.0 ± 0.0^#	90d	1.0 ± 0.0	1.4 ± 0.4	1.0 ± 0.0
180d	0.2 ± 0.2	1.4 ± 1.3	0.4 ± 0.4	180d	0.8 ± 0.2	1.6 ± 0.2**	1.5 ± 0.4 ^$
360d	0.7 ± 0.5	2.1 ± 0.2**	1.3 ± 0.5^#	360d	1.1 ± 0.4	2.3 ± 0.2**	1.9 ± 0.4 ^$

Histopathological evaluation of 2 lung lobes (left, right cranial) each stained with H&E and Sirius red 3, 7, 28, 90, 180 and 360 days after one single intratracheal instillation of PBS, DQ12 or DQ12-PVNO. For histopathological scoring, data were calculated as the sum of lesion severity and distribution. The mean of the two lobes was calculated and data expressed as mean ± sd of 5 animals per treatment and time point. DQ12 vs. PBS: * p <0.05; ** p <0.01; DQ12-PVNO vs. PBS: ^$ p < 0.05, ^$$ p > 0.01; DQ12 vs. DQ12-PVNO: # p <0.05; ## p <0.01.

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