Fig. 7

Elevated protein carbonylation in tissue lysates of rat lungs. a Tissue fragments from the left lungs of rats harvested 3 weeks after intratracheal instillation of Ag 50 PVP NP were analyzed for carbonylated proteins by 1D immune blotting. Actin served as a loading control. Control animals received 0.5 ml of vehicle (0.9 % NaCl). b Densitometric analysis of the immunoblot depicted in A). DNPH signals were summarized and adjusted to the actin signal. Animal #2 was excluded from analysis of the controls as the actin signals revealed that significantly less protein has been loaded compared to the other lanes