Table 2 DLS and RP-HPLC measurements to determine the amount of conjugated allergen
Sample | Calculated concentration from DLS (ng/ml) | Allergen molecules/AuNP determined by DLS | HPLC determined concentration (ng/ml) | Allergen molecules/AuNP determined by HPLC |
|---|---|---|---|---|
AuNP-Bet v 1 conjugates | 657 ± 13.3 | 648 ± 16 | 490 ± 68.3 | 516 ± 77 |
AuNP-Der p 1 conjugates | 578 ± 11.7 | 415 ± 10 | 530 ± 3.78 | 379 ± 2 |
AuNP-Phl p 5 conjugates | 365 ± 7.4 | 224 ± 5 | 250 ± 24.2 | 138 ± 13 |