Fig. 2

Pulmonary tissue damage measured as LDH (a) and Protein (b) in BAL of C57BL/6 mice 3 months after repeated exposure to CNC (cumulative dose of 240 μg/mouse). Black columns – female mice exposed to CNC, open columns – male mice exposed to CNC. Mean absolute values of LDH in BAL from PBS-exposed control male and female mice were 53.8 ± 2.9 U/L and 52.3 ± 2.1 U/L, respectively. Mean absolute values of protein level in BAL from PBS-exposed control male and female mice were 0.238 ± 0.005 mg/ml and 0.224 ± 0.009 mg/ml, respectively. No significant differences were found between levels of LDH and protein measured in the BAL of controls from male and female mice. Mean ± SEM (n = 10 mice/group). *p < 0.05, vs control PBS-exposed mice, ^# p < 0.05, vs male mice exposed to CNC