Lesion | Control | CNT (μg/animal) | EPOXY-REF (μg/animal) | EPOXY-CNT (μg/animal) | EPOCYL (μg/animal) | CB (μg/animal) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0 | 18 | 54 | 162 | 54 | 162 | 486 | 54 | 162 | 486 | 54 | 162 | 486 | 162 | |
Foci (small) of inflammatory cells | ||||||||||||||
Day 1 | 0/12 | 0/6 | 0/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Day 3 | 0/22 | 1/6 | 0/6 | 2/5 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 1/6 | 0/6 | 2/6 | 1/6 | 0/6 |
Day 28 | 0/24 | 2/6 | 1/6 | 1/6 | 0/6 | 0/6 | 0/6 | 1/6 | 2/6 | 2/6 | 1/6 | 2/6 | 2/6 | 0/6 |
Granuloma | ||||||||||||||
Day 1 | 0/12 | 0/6 | 0/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Day 3 | 0/22 | 0/6 | 1/6 | 1/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 | 2/6 | 2/6 | 0/6 |
Day 28 | 0/24 | 2/6 | 2/6 | 1/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 1/6 | 1/6 | 1/6 | 2/6 | 0/6 |
Polymorphonuclear cell foci | ||||||||||||||
Day 1 | 0/12 | 0/6 | 0/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Day 3 | 0/22 | 0/6 | 1/6 | 1/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 | 1/6 | 1/6 | 1/6 |
Day 28 | 0/24 | 2/6 | 1/6 | 3/6 | 0/6 | 0/6 | 0/6 | 1/6 | 1/6 | 0/6 | 1/6 | 1/6 | 2/6 | 2/6 |
Macrophages | ||||||||||||||
Day 1 | 0/12 | 2/6 | 1/5 | 1/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 | 0/6 |
Day 3 | 0/22 | 1/6 | 2/6 | 1/5 | 0/6 | 0/6 | 0/6 | 1/6 | 2/6 | 2/6 | 1/6 | 2/6 | 2/6 | 0/6 |
Day 28 | 0/24 | 1/6 | 2/6 | 2/6 | 0/6 | 0/6 | 0/6 | 0/6 | 2/6 | 2/6 | 1/6 | 1/6 | 1/6 | 0/6 |
Hyperplasia of connective tissue perivascular | ||||||||||||||
Day 1 | 0/12 | 0/6 | 0/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Day 3 | 0/22 | 0/6 | 0/6 | 1/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Day 28 | 0/24 | 0/6 | 0/6 | 1/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 |
Hyperplasia of connective tissue near bile ductless or venules | ||||||||||||||
Day 1 | 0/12 | 0/6 | 0/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Day 3 | 0/22 | 0/6 | 0/6 | 1/5 | 0/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 |
Day 28 | 0/24 | 0/6 | 1/6 | 1/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 | 1/6 | 2/6 | 2/6 |
Microfoci of necrosis | ||||||||||||||
Day 1 | 0/12 | 1/6 | 0/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Day 3 | 0/22 | 0/6 | 0/6 | 1/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 2/6 | 1/6 |
Day 28 | 0/24 | 1/6 | 2/6 | 2/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 | 1/6 | 1/6 | 1/6 |
Eosinophilic necrosis of single hepatocytes | ||||||||||||||
Day 1 | 0/12 | 0/6 | 0/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Day 3 | 0/22 | 0/6 | 1/6 | 1/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Day 28 | 0/24 | 2/6 | 1/6 | 2/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 | 1/6 | 0/6 | 1/6 | 1/6 | 1/6 |
Hepatocytes with pyknotic nuclei | ||||||||||||||
Day 1 | 0/12 | 0/6 | 0/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Day 3 | 0/22 | 0/6 | 1/6 | 1/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 |
Day 28 | 0/24 | 1/6 | 2/6 | 2/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 | 1/6 | 1/6 | 1/6 | 2/6 | 1/6 |
Parenchymatous degeneration | ||||||||||||||
Day 1 | 0/12 | 0/6 | 0/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Day 3 | 0/22 | 0/6 | 1/6 | 1/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 |
Day 28 | 0/24 | 1/6 | 2/6 | 2/6 | 0/6 | 1/6 | 0/6 | 0/6 | 1/6 | 1/6 | 0/6 | 2/6 | 2/6 | 2/6 |
Vacuolar degeneration | ||||||||||||||
Day 1 | 0/12 | 0/6 | 1/5 | 2/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 2/6 | 2/6 | 0/6 |
Day 3 | 0/22 | 2/6 | 2/6 | 3/5 | 0/6 | 0/6 | 1/6 | 0/6 | 1/6 | 2/6 | 1/6 | 2/6 | 3/6 | 2/6 |
Day 28 | 0/24 | 1/6 | 2/6 | 2/6 | 0/6 | 0/6 | 1/6 | 0/6 | 3/6 | 3/6 | 0/6 | 3/6 | 2/6 | 2/6 |
Binucleate hepatocytes | ||||||||||||||
Day 1 | 1/12 | 2/6 | 1/5 | 1/6 | 0/6 | 0/6 | 0/6 | 1/6 | 1/6 | 0/6 | 1/6 | 0/6 | 1/6 | 0/6 |
Day 3 | 1/22 | 1/6 | 2/6 | 2/5 | 1/6 | 2/6 | 1/6 | 0/6 | 1/6 | 2/6 | 1/6 | 2/6 | 1/6 | 2/6 |
Day 28 | 2/24 | 2/6 | 2/6 | 3/6 | 1/6 | 1/6 | 1/6 | 2/6 | 1/6 | 1/6 | 2/6 | 1/6 | 2/6 | 2/6 |
Oedematous endothelial cells of portal venules or close to blood vessels | ||||||||||||||
Day 1 | 0/12 | 0/6 | 0/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Day 3 | 0/22 | 0/6 | 0/6 | 1/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 | 0/6 | 0/6 | 1/6 |
Day 28 | 0/24 | 0/6 | 2/6 | 2/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 1/6 | 0/6 | 1/6 | 2/6 | 2/6 |
Increased number (hyperplasia) of Kupffer cells | ||||||||||||||
Day 1 | 0/12 | 0/6 | 0/5 | 1/6 | 1/6 | 0/6 | 1/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 |
Day 3 | 0/22 | 1/6 | 1/6 | 2/5 | 0/6 | 0/6 | 0/6 | 0/6 | 2/6 | 2/6 | 1/6 | 1/6 | 1/6 | 1/6 |
Day 28 | 0/24 | 1/6 | 2/6 | 1/6 | 0/6 | 0/6 | 1/6 | 1/6 | 1/6 | 1/6 | 1/6 | 1/6 | 2/6 | 1/6 |
Hypertrophy of Kupffer cells | ||||||||||||||
Day 1 | 0/12 | 0/6 | 2/5 | 2/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 2/6 | 2/6 | 2/6 | 0/6 |
Day 3 | 0/22 | 0/6 | 3/6 | 2/5 | 0/6 | 1/6 | 1/6 | 1/6 | 2/6 | 1/6 | 1/6 | 2/6 | 1/6 | 0/6 |
Day 28 | 0/24 | 1/6 | 2/6 | 3/6 | 0/6 | 0/6 | 1/6 | 0/6 | 1/6 | 1/6 | 0/6 | 2/6 | 2/6 | 1/6 |
Hyperplasia of bile ducts epithelium | ||||||||||||||
Day 1 | 0/12 | 0/6 | 0/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 |
Day 3 | 0/22 | 1/6 | 2/6 | 1/5 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 | 1/6 | 0/6 |
Day 28 | 0/24 | 1/6 | 1/6 | 1/6 | 0/6 | 0/6 | 1/6 | 1/6 | 1/6 | 1/6 | 2/6 | 2/6 | 1/6 | 2/6 |