Fig. 2From: The role of hypoxia-inducible factor-1α in zinc oxide nanoparticle-induced nephrotoxicity in vitro and in vivoEffects of ZnO NPs on cellular ROS and the expression of HIF-1α-related proteins in HEK-293 cells. a ROS generation in HEK-293 cells treated with ZnO NPs for 1, 3 or 5 h and with DCFH-DA for an additional 30 min. The fluorescence in the cells was immediately analyzed using a fluorescence microplate reader. b Western blotting for HIF-1α, PAI-1 and CTGF in HEK-293 cells. The cells were treated with ZnO NPs for 24 hBack to article page