Table 1 Primer and probe sequences for quantitative reverse transcription-polymerase chain reaction analyses
Gene | Sequence (5′–3′) | Tm (°C) | ||
|---|---|---|---|---|
Gapdh | (NM_008084.2) | F | TGTGCAGTGCCAGCCTCGTC | 60 |
R | GGATGCATTGCTGACAATCT | Â | ||
Sox17 | (NM_011441) | F | AAGTAGCTCCAGAAACTGCAG | 60 |
R | CTGCTCATTGTATCCATGAGGTGA | Â | ||
Tgfa | (NM_031199) | F | CTAGCGCTGGGTATCCTG | 60 |
R | CACTCACAGTGTTTGCGGAG | Â | ||
Nos3 | (NM_008713) | F | CTCTGCCTCACTCATGGGCACG | 60 |
R | GGATTTTGTAGCTCTTGTGCTGCTC | Â | ||
Tbx1 | (NM_001285472) | F | CTACCAGAATCACCGGATCACG | 60 |
R | CCGAGAGCGAGCAAAGGC | Â | ||
P | TAAGATTGCCAGCAACCCCTTCGCCAAAG | Â | ||
Kdr | (NM_010612) | F | TCACCGGAAATCTGGAGAATCAG | 60 |
R | CTCAGTACAATGCCTGAATCTTCTAC | Â | ||
P | ACAACCATTGGCGAGACCATTGAAGTGACT | Â | ||
Flt1 | (NM_010228) | F | TGAGGAGCTTTCACCGAACTC | 60 |
R | ACTCGCTATTCTCAAGTCTATCTTCA | Â | ||
P | CTTGGTCTCAGTCCAGGTGAACCGCTTC | Â | ||
Tie1 | (NM_011587) | F | CCATGCTTTTGTCTACCAAAGTCA | 60 |
R | GCGGCATTCCCAGAACCC | Â | ||
P | AGCCAGACAGGACCACAGCAGAGTT | Â | ||
Cyr61 | (NM_010516) | F | ATCGCAATTGGAAAAGGCAGC | 60 |
R | GGCGTGCAGAGGGTTGAAA | Â | ||
P | AGGCTTCCTGTCTTTGGCACCGAACC | Â | ||
Cxcl12 | (NM_021704) | F | TCGCCAGAGCCAACGTCAA | 60 |
R | GATCCACTTTAATTTCGGGTCAATGC | Â | ||
P | CCAAACTGTGCCCTTCAGATTGTTGCACGG | Â | ||