Dose-dependent induction of astrocyte activation and reactive astrogliosis in mouse brain following maternal exposure to carbon black nanoparticle

Table 4 Significantly enriched gene ontology categories according to cerebral cortex microarray data

Gene ontology	Enrichment factor	P-value	Gene symbol up-regulation
Angiogenesis	3.66	<0.001	Flt1, Kdr, Nos3, Ptprb, Robo4, Sox17, Tbx1, Tgfa, Tie1
Positive regulation of mesenchymal cell proliferation	10.11	<0.001	Foxf1, Foxp2, Kdr, Tbx1
positive regulation of epithelial cell proliferation	6.64	<0.001	Erbb2, Foxp2, Kdr, Tbx1, Tgfa
Growth factor binding	9.5	<0.001	Cyr61, Erbb2, Flt1, Kdr
Patterning of blood vessels	8.47	0.001	Cxcl12, Flt1, Lef1, Tbx1
Ventricular septum development	14.7	0.001	Cyr61, Luzp1, Stra6
Chemotaxis	4.56	0.005	Ccr3, Cmtm2b, Cxcl12, Cyr61, Flt1
Positive regulation of endothelial cell proliferation	5.7	0.005	Ccr3, Cxcl12, Kdr, Rptor
Vasculogenesis	5.7	0.005	Foxf1, Kdr, Sox17, Tie1
Blood vessel development	5.41	0.006	Foxf1, Stra6, Tbx1, Tie1
Positive regulation of cell-substrate adhesion	7.13	0.008	Cyr61, Foxf1, Nid1
Positive regulation of cell migration	3.36	0.009	Cxcl12, Cyr61, Kdr, Lef1, Flt1, Foxf1

Expression levels of 268 mRNAs were altered in a dose-dependent fashion in the high dose group versus the control group. Functional features of these 268 mRNAs were extracted using gene annotation by gene ontology (enrichment factor ≥ 2, p value ≤ 0.01, symbol number ≥ 3). Enrichment factors for each Gene ontology were defined as (nf/n)/(Nf/N), where nf is the number of flagged (differentially expressed) genes within a given category, Nf is the total number of genes within that same category, n is the number of flagged genes on the entire microarray, and N is the total number of genes on the microarray

ISSN: 1743-8977