Fig. 8
Cytotoxicity and cytokine release induced by combined exposure to stone particles and DEPB0. HBEC3-KT cells were exposed for 24 h to 25 μg/mL (2.6 μg/cm2) of DEPB0 alone or in combination with 100–200 μg/mL (10.5–21.1 μg/cm2) anorthosite (i), rhomb porphyry (ii) or quartz diorite (iii) particles. Cell viability (A) was determined using alamarBlue assay while the release of CXCL8 (B) and IL-1β (C) in the cell culture supernatants was measured using ELISA. Results are presented as boxplots of 6–7 independent experiments (Box: 25th–75th percentile, whiskers: minimum and maximum values, line: median, +: mean). Statistically significant differences were determined using two-way repeated measures ANOVA followed by Dunnett’s and Å Ãdák's post-tests. Values not adhering to model assumptions were log-transformed before statistical analysis. *Statistically significant difference from the respective control at 0 μg/mL mineral particles. #Statistically significant difference between exposure groups