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Fig. 4 | Particle and Fibre Toxicology

Fig. 4

From: Aryl hydrocarbon receptor activation-mediated vascular toxicity of ambient fine particulate matter: contribution of polycyclic aromatic hydrocarbons and osteopontin as a biomarker

Fig. 4

PM2.5 and its inorganic extract induced cell migration ability through CYP1B1 and OPN in VSMCs. VSMCs were treated with d2H2O, DMSO, 25 μg/mL PM2.5, and organic extract of PM2.5 with or without 0.1 μM TMB for 48 h. The following parameters were determined: A migration abilities of VSMCs; B OPN protein levels in the media; and C IL-6 protein levels in the media. The results are presented as mean ± SD for three independent experiments with 2–4 replicates in each experiment. *p < 0.05 compared with the Control-treated cells (n = 3, one-way ANOVA). #p < 0.05 compared with the CYP1B1 inhibitor-treated cells (n = 3, two-way ANOVA). Control: d2H2O or DMSO treatment as control. NC-VSMCs and siOPN-VSMCs were treated with d2H2O, DMSO, 25 μg/mL PM2.5, and organic extract of PM2.5 for 48 h. The following parameters were determined: D migration abilities of VSMCs; E OPN protein levels in the media; and F IL-6 protein levels in the media. The results are presented as mean ± SD for three independent experiments with 2–4 replicates in each experiment.*p < 0.05 compared with the NC-treated cells(n = 3, one-way ANOVA). #p < 0.05 compared with NC PM2.5-treated cells (n = 3, two-way ANOVA). NC: negative control, siOPN: OPN siRNA, Control: d2H2O or DMSO treatment as control

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Particle and Fibre Toxicology

ISSN: 1743-8977

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