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Fig. 5 | Particle and Fibre Toxicology

Fig. 5

From: Road tunnel-derived coarse, fine and ultrafine particulate matter: physical and chemical characterization and pro-inflammatory responses in human bronchial epithelial cells

Fig. 5

Cytokine responses in HBEC3-KT cells after exposure to PM samples from two road tunnels in comparison to stone particles from the respective tunnels. The cells were exposed to coarse, fine and ultrafine PM samples from the Marienborg (A) and Hell (B) road tunnels, respectively, during humid road surface conditions. The cells were also exposed to rhomb porphyry and quartz diorite for 20 h, at same concentration as the tunnel PM of 100 µg/mL (10.4 µg/cm2). The CXCL8 and IL-1α release were analysed by ELISA. The data represent the mean ± SEM of 5–7 experiments. *Significantly different from control, # significantly different from coarse, fine and ultrafine PM. One-way ANOVA with Tukey’s multiple comparison test, repeated measurements with p < 0.05

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