Fig. 3

Perturbation of molecular pathways in lungs of DIO and T1D mice exposed to PM. Mouse lungs were subjected to RNA sequencing to delineate biological mechanisms underlying increased susceptibility to PM-induced lung damage. DEGs obtained between DIO or T1D and ND comparisons without or with PM exposure were analyzed by IPA software for Disease and Biological Function annotation (A, C) and Canonical Pathway analysis (B, D). The results were shown as the Circo heatmaps consisting of the top 30 enriched pathways. E. Molecular regulatory network by which DIO and T1D aggravated PM-induced lung injury was illustrated derived from the transcriptome results. Pink elements and yellow arrows indicate the upregulation in DIO or T1D mice compared with ND-fed mice, while blue elements and arrows indicate the downregulation changes. F. Key genes involved in enhanced lung injury (Fig. 3E) were confirmed at the mRNA level and shown as a heatmap (n = 3)