Fig. 5

Src kinases are required to initiate Syk phosphorylation in response to SAS-NPs. Immature moDCs were pre-treated for 1 h with Saracatinib (10 µM) and then treated for 30 min with 25 µg.mL⁻¹ of SAS-NPs. A Representative experiment. B Immunoblotting of whole-cell extracts was used to quantify the phosphorylated form of Syk. The results of 3 independent experiments are represented. Bands were quantified using the Image Lab software. Results are expressed as the fold induction, representing the ratio of the normalised intensity of specific bands of treated cells divided by the normalised intensity of bands of untreated cells, *p < 0.05 One-way Anova, Kruskal–Wallis test