Fig. 1
From: Lung-gut axis of microbiome alterations following co-exposure to ultrafine carbon black and ozone
Study layout and analysis of lung inflammation. A Layout of animal exposure experiments. B Heat-map showing alterations in immune cell profile. C57BL/6J mice (8–12 weeks) were exposed to air, CB (10 mg/m3), O3 (2 ppm) or CB + O3 for 3 h for either one or four exposures and euthanized 24 h post last exposure. Data are presented as mean ± standard error of mean (SEM) and analyzed by Two-way Analysis of Variance (ANOVA) followed by Tukey’s post hoc test. Lung tissues were homogenized, stained and analyzed in LSRFortessa II. n = 3–5. *p ≤ 0.05. *denotes significantly difference from air, # denotes significantly different from O3 at the same time point, and $ denotes significantly different between single and multiple exposure groups