Fig. 4
Effect of PM2.5, the DRP1 inhibitor Mdivi-1,/ the OPA1 activator BGP-15 and DRP1 knockdown (KD)/ OPA1 overexpression (OE) on mitochondrial function in A549 cells. The effect of Mdivi-1 and BGP-15 on oxygen consumption rate (OCR) in A549 cells (A). The effect of DRP1-KD and OPA1-OE on oxygen consumption rate (OCR) in A549 cells (B). Graphical presentation of the effect of Mdivi-1 and BGP-15 on PM2.5-induced in basal respiration (C), maximal respiration (D), ATP production (E) and spare respiratory capacity (F). Graphical analysis of the effect of DRP1-KD and OPA1-OE on PM2.5-induced in basal respiration (G), maximal respiration (H), ATP production (I) and spare respiratory capacity (J). Mean values and data from individual experiments are shown. One-way ANOVA with Bonferroni’s post hoc test (for equal variance) or Dunnett’s T3 post-hoc test (for unequal variance) was performed for comparisons among multiple groups. *P < 0.05, **P < 0.01, ***P < 0.001