Fig. 5From: Single-cell transcriptome sequencing–based analysis: probing the mechanisms of glycoprotein NMB regulation of epithelial cells involved in silicosisGlycosylation level affectsGpnmb function. A. Structural domain of GPNMB protein. B. Molecular weight of GPNMB changed from 95 kD to 65 kD following treatment with 5 μg/ml of tunicamycin. C. Representative western blot results showing an action time of 16 h following treatment with 5 μg/ml of tunicamycin. D. Representative western blot results showing that the Gpnmb-OE group causes a reversal of the tunicamycin-induced elevation in α-SMA, COL1, and FN1 proteins compared to the Gpnmb-NC group. E. *P < 0.05 indicates that the α-SMA protein levels were higher in the Gpnmb-OE group than in the control group; #P < 0.05 indicates that the COL1 expression levels were lower in the Gpnmb-OE group than in the control group after tunicamycin treatment; and &P < 0.05 indicates that FN1 expression levels were lower in the Gpnmb-OE group than in the control group after tunicamycin treatmentBack to article page