Figure 6

Fibroblast proliferation is affected by exposure to bronchial epithelial cell conditioned media. Conditioned BEAS-2B media was added to a monolayer of MRC-5 fibroblast cells and DNA content was measured up to 4 days to give an indication of proliferation. A. MRC-5 proliferation is impacted by inhibition of particulate uptake in BEAS-2B cells. B. Fibroblast proliferation after exposure to conditioned media of siRNA transfected BEAS-2B cells. *indicates a statistically significant difference in DNA content compared to UC at each time point, whereas # p<0.05 indicates significant difference compared to the positive control. N= 3 for each time point in each media condition.