Figure 5

Sodium fluorescein leakage for mono-, co-, and tetraculture in transwell inserts with 0. 4, 1 and 3 μm pore size. Medium containing 10 µg/mL of sodium fluorescein was administered to the apical compartment. Medium w/o sodium fluorescein was administered to the basolateral compartment and the leaked fluorescence was measured after 60 minutes incubation at room temperature in the dark. Results obtained for inserts with a pore size of 0.4 μm, 1 μm and 3 μm are given in A, B and C, respectively. The dotted line represents the leakage of transwell inserts w/o cells. Data represents the mean of four independent transwell inserts ± SEM. Groups that are sharing the same letters are not significantly different (P > 0.05).