Table 1 Medium conditions for mono- and cocultures
Monocultures | |||
|---|---|---|---|
Cell Line | Medium | Serum | Supplements |
A549 | Dulbecco’s Modified Eagle’s Medium (DMEM) | 10% (v/v) Fetal Bovine Serum Gold |  |
THP- 1 | Roswell Park Memorial Institute (RPMI) 1640 | 25 mM HEPES; 50 μM β-mercaptoethanol | |
HMC- 1 | Iscove’s Modified Dulbecco’s Medium (IMDM) | 25 mM HEPES; 1,2 mM α-thioglycerol | |
EA. hy 926 | Dulbecco’s Modified Eagle’s Medium (DMEM) | 25 mM HEPES | |
Tetraculture | |||
75% HEPES-buffered DMEM; 15% RPMI; 10% IMDM | 10% (respectively 1%) (v/v) Fetal Bovine Serum Gold | 25Â mM HEPES | |