Figure 3

Cell mediators from SAEC exposure can pass through the Transwell membrane. SAEC were grown in the apical Transwell chamber without the presence of HMVEC in the basolateral well. HMVEC were grown in the basolaeral well without the presence of SAEC in the apical chamber. The apical chamber was exposed to DM or 1.2 μg/ml MWCNT for 24 h. Apical and basolateral media were collected for analysis by VEGFA ELISA. All values given are the mean ± standard error. SAEC only VEGFA levels increased from 89.04 ± 2.27 pg/ml to 194.04 ± 23.85 pg/ml in the apical well and 20.00 ± 2.06 pg/ml to 123.33 ± 8.13 pg/ml in the basolateral chamber. HMVEC only VEGFA levels were not above the level of detection of the ELISA assay.