Figure 5

Anakinra inhibits asbestos-induced NLRP3 and interleukin mRNA levels and secretion of cytokines in medium. Use of the IL-1ra, Ana (100 ng/mL), decreases asbestos-induced (A, B) mRNA levels of NLRP3 and IL-1β, IL-6, and IL-8 at 24 h, as quantitated by qRT-PCR (N = 3 samples/group/time point). * = significantly different (p ≤ 0.05) when compared to untreated control group (0); † = significantly different (p ≤ 0.05) when compared to asbestos group alone. Asbestos-induced secretion of IL-1β (C) IL-6 (D) G-CSF (E) and VEGF (F) were measured in LP9 cells at 24 h using a human IL-1β ELISA kit (C) or a human cytokine Bio-Plex assay (D-F) in the presence and absence of Ana (Ana = 100 ng/mL). Levels of HMGB1 (G) were determined by Western blot analysis (N = 3 samples/group). * = significantly different (p ≤ 0.05) when compared to untreated cells (0); † = significantly different (p ≤ 0.05) when compared to groups exposed to asbestos alone.