Evaluation of cytotoxic, genotoxic and inflammatory responses of nanoparticles from photocopiers in three human cell lines

Table 3 Genes and primers for gene expression analysis in THP-1 cells used in this study

Phenotypic endpoints (Stress type)	Gene	Sequence	Product size (bp)	Tm (°C)	Resource
Inflame-mation	TNF-α	F:CGAGTCTGGGCAGGTCTACTTT	299	60	UniSTS:271115
	TNF-α	R:AAGCTGTAGGCCCCAGTGAGTT	299	60	UniSTS:271115
	IL-1β	F:AGTCAGCTCTCTCCTTTCAGG	229	56	G10509
	IL-1β	R:CTTGCCCCCTTTGAATAAAT	229	56	G10509
Apoptosis	P53	F: TGTGGGATGGGGTGAGATTTC	147	60	UniSTS:151711
Apoptosis	P53	R: CTGTTGGTCGGTGGGTTG	147	60	UniSTS:151711
	CASP3	F:TATGGTTTTGTGATGTTTGTCC	195	56	G10724
	CASP3	R:TAGATCCAGGGGCATTGTAG	195	56	G10724
	CASP8	F:ATGGACTTCAGCAGAAATCTT	250	58	X98172
	CASP8	R:TCTAGTGTTTAGGTAGGTAATCAGC	250	58	X98172
DNA damage (double strand break)	KU70	F: TTTTTTTGGTTGATGCCTCC	135	60	D51189 G28341
	KU70	R: CCAAGAGATCTCGATCACTGC	135	60	D51189 G28341
	RAD51	F: TTAAAAACCTTAAGTGCTGCAGC	121	56	UniSTS: 57459
	RAD51	R: GGATTATCTTGAGTTAGTCTTAGC	121	56	UniSTS: 57459
Oxidative damage	HO1	F: CATGACACCAAGGACCAGA	155	60	[28]
	HO1	R: AGTGTAAGGACCCATCGGAG	155	60	[28]
	SOD1	F:CCATCTGTGATTTAAGTCTGGC	253	60	N32033
	SOD1	R:AAACATTCCCTTGGATGTAGTCTG	253	60	N32033
	GPX1	F: GCCTGGGCTCCCTGCGGGGCAAGG	470	60	[29]
	GPX1	R: TACGAAAGCGGCGGCTGTACCTGCG	470	60	[29]
Internal control	GAPDH	F: CCATGTTCGTCATGGGTGTGAACCA	251	60	[30]
	GAPDH	R: GCCAGTAGAGGCAGGGATGATGTTC	251	60	[30]
	18S rRNA	F: GTAACCCGTTGAACCCCATT	151	60	[31]
	18S rRNA	R: CAGGGACTTAATCAACGCAA	151	60	[31]

F: forward primer; R: reverse primer. The original primers for the selected genes were acquired through GenBank Accession number/UniSTS number or from literature.

ISSN: 1743-8977