Ambient fine particulate matter and ozone exposures induce inflammation in epicardial and perirenal adipose tissues in rats fed a high fructose diet

Table 4 Primers used for real-time PCR

Gene	Forward oligonucleotides (5’ - 3’)	Reverse oligonucleotides (5’ - 3’)	Product size (bp)
Ucp-1	TCAACACTGTGGAAAGGGACGACT	TCTGCCAGTATGTGGTGGTTCACA	119
Pgc-1α	ACGAAAGGCTCAAGAGGGACGAAT	CACGGCGCTCTTCAATTGCTTTCT	108
Dio2	TGAGGTTAAGAAGCACCGGAACCA	CGTTGGCATTATTGTCCATGCGGT	122
C/ebpβ	TGATGCAATCCGGATCAAACGTGG	TTTAAGTGATTACTCAGGGCCCGGCT	97
Cidea	ACCATCTGTTACAGGCTGTGGGAT	AAAGCTCTTGAAAGGCCCATCTGC	124
Dpt	TCCACAGTTTGAGACACATCCGGT	AGAATGCACTTTCATGCTCCACGG	126
Hoxc9	ACGCTGGAACTGGAGAAGGAGTTT	TTTGACCTGCCGCTCAGTGAGATT	102
Leptin	ATGTCACCTTGCTTTGGAAGCCAC	ATGTCCCATTGTGGGCAGTACGAT	96
Igfbp3	ACAAGGACCTTCCTTTGTCAGGCA	AAGTCATCCGGAGAACTAAGCGCA	113
Tnf-α	AGAACAGCAACTCCAGAACACCCT	TGCCAGTTCCACATCTCGGATCAT	160
Mcp-1	TGCTGTCTCAGCCAGATGCAGTTA	TACAGCTTCTTTGGGACACCTGCT	131
IL-6	GTGGAAGACAAACCATGTTGCCGT	TATTGCAGGTGAGCTGGACGTTCT	116
Adiponectin	ATACGATGTGCTTCCTGACTGGCT	TGTTGCCAGTTTCTGTGTGGATGC	125
IL-10	AGCACTGCTATGTTGCCTGCTCTT	TGACTGGGAAGTGGGTGCAGTTAT	95
β-actin	TGAGCGCAAGTACTCTGTGTGGAT	TAGAAGCATTTGCGGTGCACGATG	129

Notes: C/ebpβ CCAAT enhancing binding protein β, Cidea cell death-inducing DNA fragmentation factor-like effector A, Dio2 type 2 iodothyronine deiondinase, Dpt dermatopontin, Hoxc9 homeobox C9, Igfbp3 insulin-like growth factor binding protein 3, IL interleukin, Mcp-1 monocyte chemotactic protein-1, Pgc-1α peroxisome proliferative activated receptor gamma coactivator 1 alpha, Tnf-α tumor necrosis factor-α, Ucp-1 uncoupling protein 1.

ISSN: 1743-8977