Figure 4

Normalised cell index of cells treated with AuNPs. BEAS-2B cells (A and B), HEK 293 cells (C and D), and CHO cells (E and F) were treated with 14 nm AuNPs (left panel) and 20 nm AuNPs (right panel). BEAS-2B, HEK 293, and CHO cells were seeded and allowed to recover for 24 hours prior to treatment for 72 hours, 36 hours, or 42 hours, respectively. The slope of the curves was calculated and t-test conducted to determine differences between treated and untreated control cells. In (A) and (C), all treatments were statistically different from untreated control (p < 0.05) whilst no significant differences were observed in (B) and (D). In (E), 2 nM (p < 0.05) and 5 nM (p < 0.01) were statistically different from untreated control; whilst in (F) both 2 nM and 5nM treatments were statistically different from the untreated control at p < 0.01; n ≥ 3.