Figure 3From: Towards predicting the lung fibrogenic activity of nanomaterials: experimental validation of an in vitro fibroblast proliferation assay Influence of dispersion medium on the in vitro effect of MWCNT on fibroblast proliferation assessed by cell counting. Primary murine lung fibroblasts were treated for 24 h with different doses of NM400 dispersed in 2 or 10% FBS or 1.4 mg/ml BSA (final concentrations 0.2% FBS and 0.14 mg/ml BSA, respectively). PDGF-BB (50 ng/ml) was used as a positive control. Results are expressed relative to non-treated cell number (Ctl). * p < 0.05, ** p < 0.01 vs non-treated cells (n = 3, t-test or Dunnet multiple comparison test as appropriate).Back to article page