Figure 6

Assessment of cardiac fibrosis by Masson’s Trichrome staining. Representative images (4X objective) of sagittal sections of left ventricle free wall from mice three weeks post sham or transverse aortic constriction (TAC) surgeries (A-H), scale bar = 0.5 mm. Fibrotic regions of cardiac tissues are determined by blue staining in FA/FA (n = 5 sham, n = 5 TAC), FA/DE (n = 3 sham, n = 3 TAC), DE/FA (n = 5 sham, n = 5 TAC) and DE/DE (n = 5 sham, n = 4 TAC) groups. Assessment of percentage highly fibrotic regions was quantified (I).